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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene is noticed in many tumors, which ends up in chromosome instability, and so might lead to tumorigenesis. This protein was found to associate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a crucial position in promoting cell-cycle progression while in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, have been described. Two further splice variants ended up claimed but detailed nucleotide sequence info is just not still out there.
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Blocking peptides are peptides that bind specifically for the goal antibody and block antibody binding. These peptide usually has the epitope regarded from the antibody.
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This manual outlines further probable optimization of mobile and immunoassay parameters to be sure the best possible results are...
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This guidebook outlines even more attainable optimization of mobile and immunoassay parameters to guarantee the very best effects are...
Antibodies sure to the blocking peptide no more bind to your epitope to the focus on protein. By comparing the staining from your blocked antibody compared to the antibody alone, you can see which staining is unique.
Concentrate on Facts Cyclin E belongs on the extremely conserved cyclin spouse and children, whose customers are characterized by a remarkable periodicity in protein abundance through the mobile cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation designs which contribute to the temporal coordination of each and every mitotic function. Cyclin E types a posh with and features as being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates on the G1-S section boundary which is degraded as cells progress as a result of S period.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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